Phytochemical Screening and LC-HRMS Metabolite Profiling of Red Dragon Fruit and Barlin Banana Pseudostem from Banyuwangi: Exploring Local Natural Resources for Biomaterial Development
DOI:
https://doi.org/10.54832/phj.v8i2.1538Keywords:
Barlin banana pseudostem, LC-HRMS, Metabolomics, phytochemical screening, Red dragon fruitAbstract
Background: Banyuwangi Regency is rich in agricultural resources, including red dragon fruit (Hylocereus polyrhizus) and Barlin banana (Musa balbisiana Colla cv. Barlin), which have promising potential as renewable biomaterial sources. However, comprehensive metabolomic information on these local commodities remains limited. Objective: This study aimed to investigate the phytochemical characteristics and metabolite profiles of red dragon fruit and Barlin banana pseudostem using qualitative phytochemical screening and ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS). Methods: Both plant materials were taxonomically authenticated before extraction. Qualitative phytochemical screening was performed to detect flavonoids, phenolics, alkaloids, and saponins. Metabolite profiling was conducted using UHPLC-HRMS, and compound annotation was performed using the mzCloud™ and ChemSpider™ databases with a mass tolerance of ±5 ppm. Results: Phytochemical screening showed that the red dragon fruit extract contained flavonoids, alkaloids, and saponins, whereas the Barlin banana pseudostem extract contained only saponins. UHPLC-HRMS detected 185 and 220 chromatographic peaks in the red dragon fruit and Barlin banana pseudostem extracts, respectively. Metabolite classification identified 100 metabolites in the red dragon fruit extract and 117 metabolites in the Barlin banana pseudostem extract, predominantly comprising amino acids and derivatives, organic acids, phenolic compounds, flavonoids, alkaloids, fatty acids, terpenoids, nucleosides, vitamins, and other metabolites. Conclusion: The integration of qualitative phytochemical screening and UHPLC-HRMS provided comprehensive metabolite characterization of two important Banyuwangi agricultural resources. The generated metabolomic dataset provides a valuable scientific basis for the future development of sustainable biomaterials and other biomedical applications.
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Afshin, T., Zahri, S., Abdolmaleki, A., Asadi, A., & Eskandari, H. (2026). Magnesium-loaded carbon quantum dots in polycaprolactone scaffolds enhance angiogenesis and accelerate wound healing: In vitro, In Ovo, and in vivo evidence. Tissue and Cell, 98, 103158. https://doi.org/10.1016/j.tice.2025.103158
Ali, N. B., Adel, N., Avula, B., Katragunta, K., Tatapudi, K. K., Khan, I. A., Salama, A., Abdel-Sattar, E., & El-Shiekh, R. A. (2026). Phytochemical profiling and integrated in vitro/in vivo wound-healing evaluation of Barbeya oleoides Schweinfurth aerial parts. Journal of Ethnopharmacology, 359, 121123. https://doi.org/10.1016/j.jep.2025.121123
Al-Mekhlafi, N. A., Mediani, A., Ismail, N. H., Abas, F., Dymerski, T., Lubinska-Szczygeł, M., Vearasilp, S., & Gorinstein, S. (2021). Metabolomic and antioxidant properties of different varieties and origins of Dragon fruit. Microchemical Journal, 160, 105687. https://doi.org/10.1016/j.microc.2020.105687
Andrade, R. C., Ferreira Ribeiro, C. D., Caetano, V. C. D. S., Fernandes, S. S., & Otero, D. M. (2024). Trends in dragon fruit peel compound extraction and technological applications. Trends in Food Science & Technology, 153, 104721. https://doi.org/10.1016/j.tifs.2024.104721
Baskaran, D., Miriyam I, B., R, P., & Byun, H.-S. (2025). Effectiveness of Musa balbisiana bract toward chromium removal from industrial tannery effluent: Optimization, kinetics, isotherms, regeneration, and cost estimation. Biochemical Engineering Journal, 213, 109565. https://doi.org/10.1016/j.bej.2024.109565
Boyapati, T., Rana, S. S., & Ghosh, P. (2023). Microwave-assisted extraction of dragon fruit seed oil: Fatty acid profile and functional properties. Journal of the Saudi Society of Agricultural Sciences, 22(3), 149–157. https://doi.org/10.1016/j.jssas.2022.08.001
Carmen, F., Barthe, L., & Frances, C. (2025). Green physical processes for the extraction of organic dye betacyanin from dragon fruit (Hylocereus purpusii) peels. New Biotechnology, 85, 218–219. https://doi.org/10.1016/j.nbt.2024.08.377
Chang, V. S., Lim, L. H., Su, S. F., HuangFu, J., & Teo, S. S. (2025). The wound-healing effect of Kappaphycus alvarezii: In vitro and in vivo. Next Research, 2(2), 100246. https://doi.org/10.1016/j.nexres.2025.100246
Chauhan, B., Sharma, P., & Oberoi, D. P. S. (2026). Ultrasonic-assisted betalain extraction from red dragon fruit pulp (Hylocereus polyrhizus) using response surface methodology. Food Chemistry, 502, 147658. https://doi.org/10.1016/j.foodchem.2025.147658
Chel-Guerrero, L., Salazar-Vega, I. M., Rejón-Coral, J., Castellanos-Ruelas, A., & Betancur-Ancona, D. (2026). Techno-functional properties of unripe Musa balbisiana ABB flour for partial substitution of wheat flour in cookies and doughnuts. Food and Humanity, 6, 101107. https://doi.org/10.1016/j.foohum.2026.101107
Chelliah, A., Sundararaju, M. P., Arumugam, C., Peter, S., Suthanthiram, B., Raman, T., & Ramasamy, S. (2025). Comprehensive characterization of Nudix hydrolase genes in Musa spp.: Unveiling susceptibility factors to biotic stresses. Physiological and Molecular Plant Pathology, 139, 102749. https://doi.org/10.1016/j.pmpp.2025.102749
Chutia, G. P., Bora, S., & Phukan, K. (2024). Musa balbisiana colla banana flower derived magnetic heterogeneous nanocatalyst for cleaner biodiesel production from jatropha oil. Materials Today Sustainability, 26, 100755. https://doi.org/10.1016/j.mtsust.2024.100755
Darge, G., Asfere, Y., & Landina Lata, D. (2026). Micropropagation of two banana (Musa spp.) varieties using shoot explant from Gurage Zone, Ethiopia. Heliyon, 12(1), e44499. https://doi.org/10.1016/j.heliyon.2025.e44499
Deb, C. R., Vupru, T. N., Jamir, B., & Ngullie, T. (2026). Nutritional, physicochemical, and nutraceutical assessment of wild Musa species from Nagaland: Valorizing underutilized fruit pulp and peel. Bioresource Technology Reports, 34, 102687. https://doi.org/10.1016/j.biteb.2026.102687
Deeh, P. B. D., Han, K., & Wang, M.-H. (2025). Antioxidant, anti-tyrosinase, antidiabetic, wound healing, and cytoprotective effects of the aqueous extract of Helichrysum odoratissimum: In vitro, ex vivo, and in silico studies. South African Journal of Botany, 184, 1322–1336. https://doi.org/10.1016/j.sajb.2025.07.022
Deng, L., Liu, Q., Weng, W., & Zhang, Y. (2026). Dragon fruit peel pigment extraction residue based intelligent and biodegradable film for indicating milk freshness. Innovative Food Science & Emerging Technologies, 108, 104418. https://doi.org/10.1016/j.ifset.2025.104418
Ejegu, H., Xu, M., Kumah, C., Fentahun, B., Gebeyehu, E. K., Qingshuai, Y., Xu, G., & Shen, H. (2025). Bioactive composite nanofiber mat of PVP/PVA incorporating Clematis hirsuta for regenerative wound healing: In vitro and in vivo evaluation. Materials Today Communications, 48, 113329. https://doi.org/10.1016/j.mtcomm.2025.113329
Gao, Y., Liu, Y., Hussain, I., Wang, L., Cheng, F., Zheng, Z., Cai, Y., & Wang, X. (2026). Integrated microbiome-metabolomics profiling reveals substrate-dependent variation in agricultural Jiaosu from fruit waste. Bioresource Technology, 453, 134668. https://doi.org/10.1016/j.biortech.2026.134668
Guo, X., Qiao, J., Li, Y., Cao, H., & Kong, L. (2026). Ag/Ag-Zn IMC-Enhanced Photocatalytic and Antibacterial Properties of Cauliflower-like ZnO Nano-Composite Coatings Prepared by Plasma Spraying-Chemical Vapor Deposition. Journal of Hazardous Materials Advances, 101307. https://doi.org/10.1016/j.hazadv.2026.101307
Gurumayum, N., Devi, M. B., Khound, P., Bhattacharya, A., Sarma, H., Khan, M. R., & Devi, R. (2025). Bioactive fraction of Musa balbisiana seed mitigates D-galactose-induced brain aging via SIRT1/PGC-1α/FoxO3a activation and intestinal barrier dysfunction by modulating gut microbiota and core metabolites. Free Radical Biology and Medicine, 226, 43–55. https://doi.org/10.1016/j.freeradbiomed.2024.11.016
Hailu, L., Tesfaye, K., Mekonnen, T., Benor, S., & Guadie, D. (2026). Genetic diversity and population structure analyses of banana (Musa species) germplasm collections from Ethiopia using ISSR markers. Ecological Genetics and Genomics, 39, 100461. https://doi.org/10.1016/j.egg.2026.100461
Hanifa, N. L., Afifah, A., Wijaya, D. K., Nurmazaya, N., & Qomariyah, A. (2023). Acid and Base modified Pectin from Orange Peel as an Effective Bio-adsorbent for Pb(II) and Cr(VI) from Textile Industry Wastewater. Indo. J. Chem. Res., 10(3), 149–156. https://doi.org/10.30598//ijcr.2023.10-qom
Huo, Y., Liu, S., Huang, H., Li, Z., Ahmad, M., Zhuo, M., Li, C., Liu, B., & Li, Y. (2024). NBS-LRR genes of Musa acuminata is involved in disease resistance to Fusarium wilt. Scientia Horticulturae, 336, 113361. https://doi.org/10.1016/j.scienta.2024.113361
Jafari, A., Kolour, A. K., Shahrousvand, M., & Mohammadi-Rovshandeh, J. (2026). Development of a solvent system for electrospinning PLA/HPC/ZnO utilizing the surface response methodology for potential antibacterial wound dressing use. Heliyon, 12(11), e45078. https://doi.org/10.1016/j.heliyon.2026.e45078
Li, M., Xie, Z., Huo, K., Zeng, L., Liu, Y., Zhao, S., Jin, Z., Yang, J., Ren, L., & Hu, W. (2026). Genome-wide analyses of SNF2 chromatin remodeling complexes reveal their involvement in regulating fruit ripening in “Fenjiao” banana (Musa ABB group, cv Pisang Awak). Plant Science, 368, 113132. https://doi.org/10.1016/j.plantsci.2026.113132
Liu, Z., Wang, H., Zhang, J., Chen, Q., He, W., Zhang, Y., Luo, Y., Tang, H., Wang, Y., & Wang, X. (2024). Comparative metabolomics profiling highlights unique color variation and bitter taste formation of Chinese cherry fruits. Food Chemistry, 439, 138072. https://doi.org/10.1016/j.foodchem.2023.138072
Lopez-Nuñez, R., Lopez-Moya, F., Marhuenda-Egea, F. C., & Lopez-Llorca, L. V. (2025). Chitosan coacervates with the biocontrol fungus Pochonia chlamydosporia stimulate Musa acuminata growth and inhibit banana Fusarium wilt fungus. Carbohydrate Polymer Technologies and Applications, 11, 100990. https://doi.org/10.1016/j.carpta.2025.100990
Miranda-Salas, T. C., Olivares, B. O., León-Pacheco, R. I., Betancourt-Vásquez, M., Torrado-León, E., Angel-García, C., Gonzalez-Ulloa, A., & Rodríguez-Yzquierdo, G. A. (2026). Cosmopolites sordidus G. as a dispersal agent of Fusarium oxysporum f. Sp. Cubense Tropical Race 4 (Foc TR4) in Cavendish banana crops (Musa AAA). Fungal Biology, 130(5), 101791. https://doi.org/10.1016/j.funbio.2026.101791
Muchahary, S., Nickhil, C., Jeevarathinam, G., Rustagi, S., & Deka, S. C. (2024). Encapsulation of quercetin fraction from Musa balbisiana banana blossom in chitosan alginate solution, its optimization and characterizations. International Journal of Biological Macromolecules, 264, 130786. https://doi.org/10.1016/j.ijbiomac.2024.130786
Nguyen, K. X., Mai, H. C., Tran, T. K. N., & Nguyen, T. V. (2022). Evaluation of parameters affecting the process of extraction pectin from red flesh dragon fruit peel. Materials Today: Proceedings, 51, 1448–1454. https://doi.org/10.1016/j.matpr.2021.12.165
Novianti, C., Sari, L. D. N., Nugrahapraja, H., Suhandono, S., Dwivany, F. M., Putri, S. P., & Fukusaki, E. (2025). Metabolic profiling reveals distinctive ripening dynamics in ethylene-treated Musa balbisiana cv. ‘Pisang Klutuk Wulung’ compared to commercial Cavendish banana. Journal of Bioscience and Bioengineering, 139(4), 302–310. https://doi.org/10.1016/j.jbiosc.2025.01.001
Olivares, B. O., Calero, J., Rey, J. C., Lobo, D., Landa, B. B., & Gómez, J. A. (2022). Correlation of banana productivity levels and soil morphological properties using regularized optimal scaling regression. CATENA, 208, 105718. https://doi.org/10.1016/j.catena.2021.105718
Qomariyah, A., Nuryono, N., & Kunarti, E. S. (2021). Recovery of Gold in Au/Cu/Mg System from SH/Fe3O4@SiO2 as a Magnetically Separable and Reusable Adsorbent. Indo. J. Chem. Res., 9(1), 26–34. https://doi.org/10.30598//ijcr.2021.9-ani
Rahmana Putra, N., Nur Rizkiyah, D., Nurfaiz Mohd Faizal, A., & Hazim Abdul Aziz, A. (2024). Mini review of unlocking the hidden potential for valorization of dragon fruit peels through green extraction methods. Waste Management Bulletin, 2(2), 49–58. https://doi.org/10.1016/j.wmb.2024.03.003
Rajput, S., Sharma, D., Dutta, K. N., Roy, S., & Banerjee, S. (2025). Upcycling edible Musa balbisiana bracts waste into sustainable and possible 3D printable biomaterials. Innovative Food Science & Emerging Technologies, 104, 104156. https://doi.org/10.1016/j.ifset.2025.104156
Rangra, S., & Aggarwal, K. K. (2025). Characterization and kinetics of a cathepsin B-inhibiting protein from Musa acuminata Colla peel. Biochimie, 229, 141–150. https://doi.org/10.1016/j.biochi.2024.10.016
Rao, M. J., Wang, H., Liu, H., Tao, M., Hafeez, A., Rauf, A., Naqvi, S. A. H., Fu, X., Pan, W., Ali, Q., Duan, M., & Duan, X. (2025). Widely targeted metabolomics approach provides variation in bioactive polyphenols of strawberry fruits grown under natural environmental conditions. Plant Physiology and Biochemistry, 229, 110291. https://doi.org/10.1016/j.plaphy.2025.110291
Rawat, L. K., Bora, S., & Ghosh, T. (2026). Effect of ultrasonic, enzymatic and hybrid modification on physical, functional and thermal properties of bhimkol (Musa balbisiana) peel based dietary fiber. International Journal of Biological Macromolecules, 370, 152017. https://doi.org/10.1016/j.ijbiomac.2026.152017
Rivera-Pérez, A., Pérez-León, L., Mazzuca-Sobczuk, T., Ibáñez-González, M. J., & Garrido Frenich, A. (2026). UHPLC-Q-Orbitrap-HRMS metabolomics and molecular networking to assess non-thermal debittering of orange juice: Insights into compositional quality preservation. Microchemical Journal, 224, 117504. https://doi.org/10.1016/j.microc.2026.117504
Sarma, P. P., Das, S., Bhattacharjee, S., Khan, M. R., & Devi, R. (2026). Beneficial effects of Musa balbisiana fruit pulp and Garcinia candy on gut microbiota alterations of aged albino Wistar rats. PharmaNutrition, 35, 100471. https://doi.org/10.1016/j.phanu.2025.100471
Soraisham, R., Tongbram, P., Thingnam, S. S., Aheibam, B., Zothanzama, J., Lourembam, D. S., & Thangjam, R. (2025). Deciphering genetic relationships within and among banana (Musa spp.) genome groups using ISSR and SRAP markers. Journal of Genetic Engineering and Biotechnology, 23(3), 100540. https://doi.org/10.1016/j.jgeb.2025.100540
Thirumavalavan, M., Sukumar, K., & Sabarimuthu, S. Q. (2024). Trends in green synthesis, pharmaceutical and medical applications of nano ZnO: A review. Inorganic Chemistry Communications, 169, 113002. https://doi.org/10.1016/j.inoche.2024.113002
Veer, S. J., Pawase, P. A., Khalid, S., Dattatraya, R. H., Bashir, O., & Abdi, G. (2026). Pectinase-assisted extraction of red dragon fruit (Hylocereus polyrhizus) juice: Process optimization and quality assessment. Food Chemistry Advances, 10, 101244. https://doi.org/10.1016/j.focha.2026.101244
Vupru, T. N., & Deb, C. R. (2026). Genetic differentiation of wild Musa accessions using inter-simple sequence repeat and sequence-related amplified polymorphism. South African Journal of Botany, 193, 333–342. https://doi.org/10.1016/j.sajb.2026.04.039
Wang, J., Yu, Z., Yu, J., Feng, Y., Ye, C., Tong, X., Huang, Y., Wang, T., Zhu, L., Li, Y., Lin, J., Wen, C., & Ma, J. (2026). Myrrh-loaded superhydrophobic gauze effectively treats wound infections and promotes healing: In vitro and in vivo efficacy. Biomaterials Advances, 180, 214538. https://doi.org/10.1016/j.bioadv.2025.214538
Wang, Y., Ali, M., Teng, F., Shi, C., Zhang, X., Li, F., Ji, N., & Li, X. (2026). Integrated metabolomic and mRNA transcriptomic analyses reveal key metabolites and genes involved in browning of fresh-cut pitaya. International Journal of Biological Macromolecules, 366, 152444. https://doi.org/10.1016/j.ijbiomac.2026.152444
Yan, Y., Li, X., & Xiong, W. (2026). Chitosan-ZnO/g-C3N4 composite for effective light/dark antibacterial activity via dual-mode action of contact killing and ROS generation. Journal of Environmental Chemical Engineering, 14(3), 122691. https://doi.org/10.1016/j.jece.2026.122691
Zhou, R., Wang, S., Zhan, N., He, W., Deng, G., Dou, T., Zhu, X.-T., Xie, W.-Z., Zheng, Y.-Y., Hu, C., Bi, F., Gao, H., Dong, T., Liu, S., Li, C., Yang, Q., Wang, L., Song, J.-M., Dang, J., … Sheng, O. (2024). High-quality genome assemblies for two Australimusa bananas (Musa spp.) and insights into regulatory mechanisms of superior fiber properties. Plant Communications, 5(1), 100681. https://doi.org/10.1016/j.xplc.2023.100681
